Composite
Part:BBa_K1997012:Design
Designed by: Chushu Zhu Group: iGEM16_NUDT_CHINA (2016-10-13)
P+R->sHRP-N->Zif268-> sHRP-C->TER
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 1171
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 1174
Illegal BsaI.rc site found at 907
Illegal SapI.rc site found at 1288
Design Notes
We added BsaI sites at the flank of "Zif268" sequence so that this sequence can be easily replaced
Source
This part was synthesized by IDT